Alzheimer’s disease (AD) is a progressive neurodegenerative disorder with an increasing prevalence. Although well-studied, there are still many questions about the origin of AD. Recently, it became evident that inflammation, both peripheral and central, plays an important role. This increase in knowledge can be attributed to the rejection of the long-held belief that the brain is an immune-privileged site. By now it is becoming increasingly clear that immune cells play an important role in both healthy and diseased brains. In shear contrast to the now well-studied interaction between the innate immunity and AD pathology, the knowledge of the role of the adaptive immune system is scarce. In this project, I aim to unravel the interplay between AD and the different T cell subsets. I intend to identify different subsets in AD setting by single-cell sequencing and determine their localization in the mouse brain through high-dimensional flow cytometry and confocal imaging. In addition, I will investigate the effect of the identified T cell subsets on AD pathology, more specifically the effect on microglia, astrocytes and neuronal cells. I will do this via independent approaches, by using OPAL multiplex immunohistochemistry staining, co-cultures of the relevant cells and adoptive transfers. Ultimately, this knowledge will be indispensable for the rational design of an immunomodulatory therapy against AD that fine-tunes specific parts of the immune system.