Code
31511411
Duration
01 January 2011 → 16 September 2012
Funding
Research Foundation - Flanders (FWO)
Promotor
Research disciplines
-
Natural sciences
- Biochemistry and metabolism
- Systems biology
-
Medical and health sciences
- Medical biochemistry and metabolism
- Medical biochemistry and metabolism
- Medical biochemistry and metabolism
Keywords
MAPPIT
HIV-1
protein interactions
Project description
This project uses MAPPIT (Mammalian Protein-Protein Interaction Trap)to study known interactions between HIV-1 and host proteins and identifies new interactions between the two. We use a MAPPIT-based mutagenesis approach and a newly developed MAPPIT-based screening strategy to map the interaction domain for a number of protein interactions including Apobec3G-Vif and Apobec3G-gag. Array MAPPIT allows us to screen for new protein interactions.