Granzyme-mediated cell-targeted and antiviral methods-of-action: a targeted proteomic analysis

01 October 2008 → 30 September 2014
Regional and community funding: Special Research Fund, Research Foundation - Flanders (FWO)
Research disciplines
  • Natural sciences
    • Biochemistry and metabolism
    • Systems biology
  • Medical and health sciences
    • Medical biochemistry and metabolism
    • Medical biochemistry and metabolism
    • Medical biochemistry and metabolism
substrates protease N-terminal proteomics
Project description

The COFRADIC or 'COmbined FRActional DIagonal Chromatography' technology specifically isolates peptides holding the amino terminal part of proteins. Since protein processing gives rise to a new protein fragment, isolation of N-terminal peptides points to proteolytic event and permit analysis of protein processing on a proteome-wide scale. The proteome-wide degradomics methods will be applied to study granzyme-mediated target and host-virus interactions.