Cupriavidus necator is a chemolithoautotrophic microorganism that naturally produces and accumulates significant amounts of polyhydroxyalkanoates (PHAs), a class of bioplastics. In addition, this bacterium can fix and assimilate CO₂ into biomass and other carbon-based compounds. These features make C. necator a promising platform for the sustainable production of biofuels and bioplastics. However, the development of this organism as an industrial chassis remains hindered by the lack of efficient genetic engineering tools. Therefore, the foundation of this doctoral research lies in the development of novel methods for targeted genetic deletions and integrations. Using these newly established tools, several engineered strains will be constructed for the sustainable production of (R)-1,3-butanediol, poly(3-hydroxybutyrate-co-3-hydroxypropionate), and fatty alcohols.