Lactobacillus species in gut microbiota shows great promise in alleviation of hyperuricemia (HUA) disease. However, little is known about the melecular mechanism of how Lactobacillus interacts with uric acid (urate) in circulation. Here, we investigated the improvement in systemic urate metabolism by oral administration of Lactobacillus based on the both of in vitro and in vivo experiments. At first, the Lactobacillus with ability of nucleosides degradation were isolated from Chinese fermented vegetables. Their probiotic properties including tolerance to simulated gastroinestinal tract, colonization and bacteriostatic ability were then compared for selecting one strain of Lactobacillus with great potential as the anti-HUA probiotic, which was confirmed as Lactiplantibacillus plantarum (L. plantarum) FS4722  strain. Subsequently, the anti-HUA function of L. plantarum FS4722 was verified in HUA mice, as evidenced by the significant decrease of serum urate resulted by the inhibition of urate synthesis in liver. Finally, the beeswax/ethycelluloase oleogels were fabricated as encapsulant to protect the L. plantarum FS4722 against the harch conditions. This study would pave the way for the application of Lactobacillus probiotics in the HUA prevention and management.