Code
3F014111
Duration
01 October 2011 → 22 January 2013
Funding
Regional and community funding: Special Research Fund, Research Foundation - Flanders (FWO)
Promotor
Fellow
Research disciplines
-
Natural sciences
- Genetics
-
Medical and health sciences
- Cardiac and vascular medicine
- Gynaecology and obstetrics
- Molecular and cell biology
- Morphological sciences
- Cardiac and vascular medicine
- Gynaecology and obstetrics
- Molecular and cell biology
- Morphological sciences
- Cardiac and vascular medicine
- Gynaecology and obstetrics
- Molecular and cell biology
- Morphological sciences
Keywords
directed differentiation
recombination mediated cassette exchange (RMCE)
reprogramming
mouse model
cardiomyocytes
induced pluripotent stem cells (iPSCs)
Project description
In this project we propose to create a ROSA26 locus based ‘iPS’ mouse line that
would allow for conditional and inducible (COIN) cell-specific as well as temporally-regulated cellular
reprogramming. This model will be created in such a manner that it allows for deletion and exchange of the
reprogramming factors (RF’s) via a recombinase mediated cassette exchange (RMCE) strategy. By exchanging the
RF’s by lineage specific key transcription factor(s) we will re-differentiate the formed iPSCs toward a specific
lineage, like cardiomyocytes, which can be used for cardiac repair studies in vivo.