Project

Generation and characterization of the “ROSA26-iPS mouse”: a conditional, inducible and exchangeable resource to study cellular (de)differentiation

Code
3F014111
Duration
01 October 2011 → 22 January 2013
Funding
Regional and community funding: Special Research Fund, Research Foundation - Flanders (FWO)
Promotor
Research disciplines
  • Natural sciences
    • Genetics
  • Medical and health sciences
    • Cardiac and vascular medicine
    • Gynaecology and obstetrics
    • Molecular and cell biology
    • Morphological sciences
    • Cardiac and vascular medicine
    • Gynaecology and obstetrics
    • Molecular and cell biology
    • Morphological sciences
    • Cardiac and vascular medicine
    • Gynaecology and obstetrics
    • Molecular and cell biology
    • Morphological sciences
Keywords
directed differentiation recombination mediated cassette exchange (RMCE) reprogramming mouse model cardiomyocytes induced pluripotent stem cells (iPSCs)
 
Project description

In this project we propose to create a ROSA26 locus based ‘iPS’ mouse line that

would allow for conditional and inducible (COIN) cell-specific as well as temporally-regulated cellular

reprogramming. This model will be created in such a manner that it allows for deletion and exchange of the

reprogramming factors (RF’s) via a recombinase mediated cassette exchange (RMCE) strategy. By exchanging the

RF’s by lineage specific key transcription factor(s) we will re-differentiate the formed iPSCs toward a specific

lineage, like cardiomyocytes, which can be used for cardiac repair studies in vivo.