Project

Development of novel expression technology for eukaryotic membrane proteins: application to G-protein coupled receptors

Duration
01 October 2010 → 30 September 2014
Funding
Regional and community funding: Special Research Fund, Research Foundation - Flanders (FWO)
Research disciplines
  • Natural sciences
    • Biochemistry and metabolism
    • Systems biology
  • Medical and health sciences
    • Medical biochemistry and metabolism
    • Medical biochemistry and metabolism
    • Medical biochemistry and metabolism
  • Engineering and technology
    • Biomaterials engineering
    • Biological system engineering
    • Biomaterials engineering
    • Biomechanical engineering
    • Other (bio)medical engineering
    • Environmental engineering and biotechnology
    • Industrial biotechnology
    • Other biotechnology, bio-engineering and biosystem engineering
Keywords
nanobody GPCR yeast engineering
 
Project description

(1) Integration of several engineering steps for the optimalisation of GPCR overexpression in engineered yeast strains with increased ER-membrane capacity and use of an existing (T4 lysozyme insertion) and a new (nanobody complexation by coëxpression) method for GPCR-stabilisation and crystallisation (2) Production, extraction, purification and crystallization of the GPCR membrane proteins