Research Explorer

Project

‘VIROTRAP’: A new strategy for the detection of protein-protein interactions
Acronym
VIROTRAP
Code
178GE0811
Duration
01 October 2010 → 05 January 2016
Funding
Regional and community funding: IWT/VLAIO
Research disciplines
  • Natural sciences
    • Biochemistry and metabolism
    • Systems biology
  • Medical and health sciences
    • Medical biochemistry and metabolism
    • Medical biochemistry and metabolism
    • Medical biochemistry and metabolism
  • Engineering and technology
    • Biomaterials engineering
    • Biological system engineering
    • Biomaterials engineering
    • Biomechanical engineering
    • Other (bio)medical engineering
    • Environmental engineering and biotechnology
    • Industrial biotechnology
    • Other biotechnology, bio-engineering and biosystem engineering
Keywords
interactomics technological development p55 Gag
 
Project description
 

This project aims for the introduction, optimization and application of a new technology based on virus biology for the detection of protein interactions. In this VIROTRAP-technology, we couple a bait protein to the p55 Gag protein of HIV-1. This fusion protein forms ‘virus like particles’ which ‘trap’ endogenous interaction partners and can be harvested from the cell supernatant, after which we identify the interaction partners by e.g. mass spectrometry.