Universal antibody scaffolds with drug-like features for straightforward mini-domain grafting

01 February 2022 → Ongoing
Regional and community funding: Special Research Fund
Research disciplines
  • Medical and health sciences
    • Drug discovery and development not elsewhere classified
    • Biopharmaceuticals
    • Biopharmaceutics
biotherapeutics antibodies protein biophysics
Project description

In this project, I will investigate whether mini-domain grafting affects favourable physicochemical properties of antibodies (also known as drug-like properties). I will achieve this by designing a set of model proteins, assessing their expression in production cell lines, and applying a wide range of techniques to analyze the structure, stability and antigen-binding properties of the purified chimeric proteins in comparison to the wild-type antibodies. The project goal is to define the potential of grafting mini domains onto antibodies and to find the most suitable antibody scaffolds for this approach. The findings of the project could potentially lead to the development of a platform approach for the straightforward design of novel biotherapeutic proteins.