Flemish citizens are chronically exposed to dietary & environmental contaminants, such as mycotoxins & persistent organic pollutants (POPs). Mycotoxins are natural toxic fungal metabolites present in food worldwide & POPs are chemicals that persist in nature. Severe health effects are linked with human exposure to these contaminants, e.g. increased cancer risk & endocrine disruption, with young people being especially vulnerable to the latter. Human biomonitoring (HBM) allows for accurate assessment of exposure at the individual level, which can be integrated with dietary data to identify the source of exposure. Minimally invasive techniques for blood sampling are preferred, e.g. volumetric absorptive microsampling (VAMS), due to their inherent advantages. There is also growing interest in automation via robotics to increase analysis throughput. In the MICRO-HBM project, automated VAMS-based HBM methods for mycotoxin & POP biomarkers will be developed & applied in the Flemish Environment & Health Studies (FLEHS)-V cohort (n≥300) on Flemish adolescents. Reference values will be established to identify subgroups with higher exposure and to compare with HBM-guidance values. In addition, serum samples of previous FLEHS-cycles will be analysed (n=1,200) for time series analysis to assess the effectiveness of public health efforts to reduce exposure & the impact of climate change on mycotoxin exposure.