Research Explorer

Project

Minimizing metabolic burden caused by heterologous expression of synthetic pathways: development of sensors & actuators, implementation of a genetic circuit with beta-carotene production in E. coli as case study
Code
3F025013
Duration
01 October 2013 → 30 September 2017
Funding
Regional and community funding: Special Research Fund, Research Foundation - Flanders (FWO)
Research disciplines
  • Natural sciences
    • Synthetic biology
  • Engineering and technology
    • Industrial microbiology
Keywords
Escherichia coli metabolic engineering genetic circuit fine tuning expression synthetic biology metabolic burden
 
Project description

The expression of synthetic multi-gene pathways in non-native production hosts often results in metabolic burden, which is detrimental for productivity. A novel genetic circuit will be developed which specifically controls the expression of the heterologous pathway, so that metabolic burden can be avoided. This novel synthetic biology tool will be validated using beta-carotene production in E. coli as case study.