The goal of this project is the automatisation of a set of chemical and cleavage and derivatisation methods for proteins, developed at the laboratory for protein biochemistry and protein engineering. These methods allow a better characterisation of proteins in proteomic projects using MALDI TOF/TOF MS analysis. Applications cover an improved characterization of C-terminal processing of human proteins. Automatization will allow to dramatically increase the number of samples per day as well as significantly reduce risks of contamination.