Project

Study of the essential function of beta-actin in embryonic development and the necessity for correct localisation of beta-actin MRNA during neuronal development

Duration
01 October 2007 → 01 October 2008
Funding
Regional and community funding: Special Research Fund
Research disciplines
  • Natural sciences
    • Animal biology
    • Biochemistry and metabolism
    • Genetics
    • Systems biology
  • Medical and health sciences
    • Medical biochemistry and metabolism
    • Morphological sciences
    • Medical biochemistry and metabolism
    • Morphological sciences
    • Medical biochemistry and metabolism
    • Morphological sciences
  • Agricultural and food sciences
    • Veterinary medicine
Keywords
actin isoforms embryonic development cytoskeleton
 
Project description

Through analysis of the B-actin knock-out en knock-in mice, and derived cell lines thereof, we will study the essential functions of B-actin in development (more specific in vascularisation and neuronal differentiation) and the role of the 3'UTR in correct localization and function of B-actin. Several methods will be used, including in situ immunostaining, primary cell cultures, RNAi and differential proteome analysis.