Recent technological advances have improved our understanding of complex living systems, such as
humans, animals, and crops, by studying their genetic information and how it is expressed. By
examining genes and molecules in individual cells, we have discovered cellular variations and even
identified previously unknown cell types.
However, current technology has limitations. It lacks sensitivity, potentially missing important
information, and it is expensive and time-consuming to use. Moreover, exciting applications like
mapping genetic changes in cancer or studying specific DNA modifications are not supported by
existing tools.
To address these challenges, we propose acquiring a new equipment that can produce customized
materials for genetic analysis. This equipment, called a photolithographic printer, can create
thousands of different DNA molecules at high resolution. These molecules can be used to fabricate
solid surfaces with specific DNA patterns, enabling detailed genetic analysis of tissues. Alternatively,
the DNA molecules can be released from the solid surface for solution-based analysis.
This technology allows us to study large tissue areas and analyze genetic information at single-cell
level. It also enables research on organisms lacking appropriate tools. Additionally, it facilitates the
study of different RNA molecules, including those that are typically overlooked but may have
important functions, such as non-polyadenylated long non-coding RNAs.