Research Explorer

Project

Development of C-terminal peptide-based proteome-analytical strategies and the extensive mapping of the granzyme substrate-repertoire
Code
31510710
Duration
01 January 2010 → 31 December 2012
Funding
Research Foundation - Flanders (FWO)
Research disciplines
  • Natural sciences
    • Biochemistry and metabolism
    • Systems biology
  • Medical and health sciences
    • Medical biochemistry and metabolism
    • Medical biochemistry and metabolism
    • Medical biochemistry and metabolism
Keywords
substrates protease cofradic
 
Project description
 

This project involves the development of proteome-wide strategies for the isolation of C-terminal peptides. As such, the sample complexity is reduced to a maximum since each final protein is represented by a single peptide. By the combined use of N-and C-terminal COFRADIC, one can obtain a full proteome coverage, thereby enabling the extensive mapping of protease substrate-repertoires.