Industrial biotechnology puts microorganisms such as bacteria, yeasts or molds to work for the production of valuable compounds. In this way, chemicals, food or feed ingredients, detergents and biofuels are mass-produced by microorganisms in an eco-friendly manner. Very often, molecular biology is applied to alter the DNA of these microorganisms in order to enlarge the range of interesting compounds they produce by nature. However, assembly of these compounds usually occurs inside the cell, bringing along problems regarding toxicity and reduced yields. Moreover, in quite some cases problems are encountered to get them out of the cell. This PhD proposal is designed to solve this problem with the CROSS OVER system. The compound of interest will be tagged with a simple but specific molecule: the vehicle. Next, this vehicle will escort the compound outside the cell by use of a transporter protein. Once outside the cell, the vehicle will be detached and will be re-shuttled into the cell to be reused for another round of
export. With this system, the interesting compounds are collected outside where much more space is available and their recovery is easier. As a result, the production yield of valuable compounds will be largely increased.