Multiplexed cytokine and chemokine analysis for the quantification of single or multiple immunotargets in biofluids is pivotal for the diagnosis and management of patients with systemic inflammatory disease. It allows early diagnosis of complex diseases, longitudinal monitoring and quantification of disease activation before and after targeted treatment, functional validation of novel disease-causing genes and finally the identification of novel disease biomarkers unravelling the basic molecular underpinning of systemic inflammatory diseases. Such analyses require high sensitivity and specificity across a large dynamic range. MSD QuickPlex SQ 120 instrument offers high-performance electrochemiluminescence (ECL) immunoassays with a great precision, high sensitivity and less inter-assay variation.