Yeast cultivations will be performed in a specifically designed continuous bioreactor. An integrative-experimental approach will be used to assess the effect of microgravity. Therefore, various omics technologies, i.e. fluxomics, transcriptomics, proteomics and genomics, and specific cell analysis methods will be used to analyse the samples. A network biology model for S. cerevisiae will be set-up to process the omics data. This will lead to insight into how gravity influences global regulation of energy metabolism, (stress) signaling transduction pathways, transcriptional regulatory networks, gene regulatory networks, protein-protein interaction networks, and metabolic networks.