RAPP-ID ("Development of RApid Point-of-care test Platforms for Infectious Disease") will develop a Point-of-Care Test (POCT) for rapid (hospital <2h primary care><30 min) detection of bacteria mycobacteria fungi as well viruses and host biomarkers by combining novel specific probes methods sample preparation demonstrated ultra-high sensitive methods. The platforms will also determine resistance to antimicrobial drugs. research on the pathogens involved in 1) Sepsis 2) Lower Respiratory Tract Infections (LRTI) including Community-Acquired Pneumonia (CAP) Ventilator-Associated (VAP) 3 Tuberculosis (TB).>

RAPP-ID (“Development of RApid Point-of-Care test Platforms for Infectious Diseases”) will
develop a Point-of-Care Test (POCT) for rapid (hospital <2h, primary care <30min) detection of
bacteria, mycobacteria, fungi, as well as viruses and host biomarkers by combining novel specific
probes, novel methods of sample preparation, and demonstrated ultra-high sensitive detection
methods. The platforms will also determine resistance to antimicrobial drugs. The research will focus
on the biomarkers and pathogens involved in 1) Sepsis, 2) Lower Respiratory Tract Infections (LRTI),
including Community-Acquired Pneumonia (CAP) and Ventilator-Associated Pneumonia (VAP), and
3) Tuberculosis (TB). Detection of bacteria, fungi and antibiotic resistance will mainly involve
Nucleic Acid (NA) tests, whereas viral and host biomarker detection will mainly involve selective
immunobinding with a probe or with a sensor surface. The diagnostic tests consist of in four
functional modules: 1) sample collection and interfacing, 2) upconcentration and extraction, 3) signal
and/or sample amplification, and 4) detection. RAPP-ID will integrate the minimum number of
modules required for each disease/syndrome in a microfluidic cartridge, for which a breadboard reader
with Graphical User Interface (GUI) provides the necessary optical/fluidic/electric/thermal interfaces.
The integrated POCT will be validated on (spiked) reference samples and well characterised clinical
samples and compared with the best reference standards and other standard available diagnostic tests.