Endogenous tagging of scaffold proteins: profiling switches in signal transduction pathways

01 January 2014 → 31 December 2016
Research Foundation - Flanders (FWO)
Research disciplines
  • Medical and health sciences
    • Medical imaging and therapy
    • Medical imaging and therapy
    • Medical imaging and therapy
primary pathways stem cells
Project description

The manipulation of the genome was mainly restricted to mouse stem cells. REcent advancements have made it possible to 'engineer' the genomes of cell lines, including those of human origin. In this project, we will use one of these novel technologies to introduce purification and detection tags at the end of proteins by altering the coding sequence in the genome. We will focus on scaffold proteins, which are typically structural proteins that provide a platform for the integration of different signal pathways. The research budget will also be used to characterize our model system extensively by use of next generation sequencing. This is critical for both the succesful design of engineering tools and for the analysing of data afterwards.