Research Explorer

Project

Accurate peptide/protein quantification as a quality control and normalization template for biological proteome samples prior to LC-MS/MS using the Big Lunatic microfluidic UV/vis system.
Code
01B12620
Duration
01 December 2020 → 30 November 2022
Funding
Regional and community funding: Special Research Fund
Research disciplines
  • Natural sciences
    • Analytical spectrometry
    • Spectroscopic methods
    • Analytical biochemistry
    • Proteins
    • Proteomics
Keywords
Proteomics accurate quantification normalization quality control peptides LC-MS/MS epigenetics histones post-translational modifications
 
Project description
 

In the proteomics research field proteome quantification is often used to normalize biological samples before LC-MS/MS analysis. Current techniques available are limited and quite inaccurate (SDS-PAGE / Coomassie), all the more so because quantification has to be done before sample preparation. The Big Lunatic enables accurate peptide quantification preceding the LC-MS/MS analysis, leading to higher quantitative accuracies.