The main objective of this research is twofold. On the one hand it aims for an efficient biotechnological production of human milk sugars (HMOs), which have a clear positive effect on a baby's health and development when added to formula milk. On the other hand, RNA engineering will be investigated as a novel engineering tool. By enhancing the stability of specific transcripts, a lower mRNA turnover rate will be obtained which decreases the need for a high transcription rate and the metabolic burden associated with it.