Bacterial respiratory infections in poultry present significant economic challenges to the poultry industry worldwide. Management of these infections is crucial for maintaining healthy poultry, ensuring food safety, and meeting the growing global demand for poultry products. Control and treatment of bacterial diseases in poultry have relied on antibiotics. However, this led to antimicrobial resistance. Consequently, there is an urgent need for alternatives to antibiotics to mitigate bacterial respiratory infections in poultry. We engineered a novel antimicrobial compound and in this project it will be validated in vitro in chicken lungs cells and in vivo in experimentally infected specific pathogen free chickens. Simultaneously, vaccines have played a pivotal role in preventing bacterial respiratory infections. To address the limitations of the currently used vaccines, we designed a next generation vaccine and succesfully tested it in vitro. In this project the efficacy of the vaccine will be validated in infected SPF chickens.