This project aims to develop thiolated nanofibers (NFs) using non-thermal plasma polymerization Surprisingly, to date, no knowledge on atmospheric pressure plasma deposition of thiol-rich coatings on NFs is available, while this technology offers several advantages, including dry processing and fiber morphology preservation To fully examine the potential of thiol plasma polymerization, 4 precursors (1-propanethiol, 1,3-propanedithiol, thiophenol and 2-(mercaptoalkyl)-2-oxazoline) will be used As we aim to conduct a largely fundamental study that will serve as a model for future thiol plasma chemistry research, the following strategy is proposed: for each precursor, the effect of various plasma parameters on the properties of the deposited coatings will be performed In parallel, the gas phase will be probed as a function of these parameters to reveal correlations between the plasma gas phase chemistry and the properties of the thiol-rich coatings, which, in a third step, will be fed into a 0D kinetic model as well as a 1D CFD model, permitting fundamental insights into the chemistry and acquiring a superior control over the deposition process In the final step of the project, the capacity to immobilize fibronectin will be studied and cell-surface interactions on thiolated and protein-immobilized NFs will be examined If successful, these novel coatings can realize a major breakthrough in thiol plasma chemistry research