Project

A new fragment-centric perspective on proteomics

Code
3I008822
Duration
01 May 2022 → 30 April 2026
Funding
Research Foundation - Flanders (FWO)
Research disciplines
  • Natural sciences
    • Development of bioinformatics software, tools and databases
    • Structural bioinformatics and computational proteomics
    • Epigenetics
  • Medical and health sciences
    • Protein diagnostics
Keywords
histone code proteoforms Mass spectrometry tissue leakage proteins data-independent acquisition
 
Project description

This application is situated in the field of mass

spectrometry-based (MS) proteomics. Our consortium

brings together unique N-terminomics technologies from

the Gevaert lab with the expertise on histone epigenetics

and on new Q-TOF acquisition strategies from

ProGenTomics (Prof. Deforce and Dr. Dhaenens), the

machine learning based identification algorithms developed

at the Martens lab and statistical solutions for robust

quantification and differential analysis from the Clement

lab. Together, our labs span the full proteomics workflow

from biological sample collection and processing, over data

acquisition to data analysis. At the heart of our proposal lies

a novel mass spectrometric design from SCIEX, the ZenoTOF

7600 system which combines a novel, game changing

fragmentation technology (Electron Activated Dissociation

or EAD) with the Zeno trap, which increases instrument

sensitivity ten-fold. The latter is especially relevant in the

context of the recently published but not yet commercially

available Scanning SWATH data-independent acquisition

(DIA) strategy for extremely high-throughput proteomics,

which has been beta-tested on an earlier instrumental

design (SCIEX TripleTOF 6600+) at ProGentomics since

summer 2020. Markedly, the ZenoTOF was launched in the

summer of 2021 using the quote of M. Dhaenens: Zeno

opens the door to a fragment centric revolution