This application is situated in the field of mass spectrometry-based (MS) proteomics. Our consortium brings together unique N-terminomics technologies from the Gevaert lab with the expertise on histone epigenetics and on new Q-TOF acquisition strategies from ProGenTomics (Prof. Deforce and Dr. Dhaenens), the machine learning based identification algorithms developed at the Martens lab and statistical solutions for robust quantification and differential analysis from the Clement lab. Together, our labs span the full proteomics workflow from biological sample collection and processing, over data acquisition to data analysis. At the heart of our proposal lies a novel mass spectrometric design from SCIEX, the ZenoTOF 7600 system which combines a novel, game changing fragmentation technology (Electron Activated Dissociation or EAD) with the Zeno trap, which increases instrument sensitivity ten-fold. The latter is especially relevant in the context of the recently published but not yet commercially available Scanning SWATH data-independent acquisition (DIA) strategy for extremely high-throughput proteomics, which has been beta-tested on an earlier instrumental design (SCIEX TripleTOF 6600+) at ProGentomics since summer 2020. Markedly, the ZenoTOF was launched in the summer of 2021 using the quote of M. Dhaenens: “Zeno opens the door to a fragment centric revolution”