Pseudomonas-derived Cyclic Lipodepsipeptides (CLiPs) showcase antibacterial, antifungal, and cytotoxic potential for agriculture and health applications. The study aims to deepen understanding of their bioactivity by investigating the role of the inherent acyl chain and enhancing potency through inclusion of additional chains. Utilizing total synthesis, NMR structure determination, molecular modelling, and bioactivity assessments, the project aims to establish superior structure-activity relationships.