Combined dual transcriptomic, proteomic and functional investigations to reveal host-pathogen interactions behind the virulence of foodborne pathogen Bacillus cereus

01 January 2022 → 30 June 2022
Regional and community funding: Special Research Fund
Research disciplines
  • Natural sciences
    • Transcription and translation
  • Agricultural and food sciences
    • Food microbiology
transcriptome RNAseq bacillus cereus toxins virulence host-pathogen interaction transport proteomics microaaray transwell Seahorse cytotoxicity
Project description

A toxin-mediated infection of foodborne Bacillus cereus requires direct contact of viable B. cereus with its host for in-situ production of diarrheal enterotoxins. This direct contact leads to a cross-talk between a pathogen and its host. The interaction will be investigated by functional, cytotoxic and
transcriptomic approaches, using tools such as Seahorse flux analysis, RNAseq, microarrays, RTPCR, ELISA and mass-spectrometry.