Acronym
Licht in het donker
Code
01D28019
Duration
01 October 2019 → 01 November 2019
Funding
Regional and community funding: Special Research Fund
Promotor
Fellow
Research disciplines
-
Medical and health sciences
- Cell signalling
- In vitro testing
- Analytical toxicology
Keywords
New Psychoactive Substances
GPCR
Biased signaling
Luminescence
Multiplex
Screening
Bioassay
Functional Complementation
Nanoluciferase
Click Beetle Luciferase
Project description
We present an innovative bioassay that casts light upon the pharmacology and toxicity of new psychoactive substances (NPS). By coupling distinct signaling pathways to the functional complementation of two luciferases, different colors of light are emitted. Secondly, this project aims at multiplexed NPS detection. We propose an activity-based assay in which color-shifted nanoluciferases generate distinctly colored light upon receptor activation.