Project

Leave a light on: towards multiplexed luminometric characterization and detection of new psychoactive substances.

Acronym

Licht in het donker

Code

01D28019

Duration

01 October 2019 → 01 November 2019

Funding

Regional and community funding: Special Research Fund

Promotor

Christophe Stove

Fellow

Marthe Vandeputte

Research disciplines

Medical and health sciences
- Cell signalling
- In vitro testing
- Analytical toxicology

Keywords

New Psychoactive Substances GPCR Biased signaling Luminescence Multiplex Screening Bioassay Functional Complementation Nanoluciferase Click Beetle Luciferase

Project description

We present an innovative bioassay that casts light upon the pharmacology and toxicity of new psychoactive substances (NPS). By coupling distinct signaling pathways to the functional complementation of two luciferases, different colors of light are emitted. Secondly, this project aims at multiplexed NPS detection. We propose an activity-based assay in which color-shifted nanoluciferases generate distinctly colored light upon receptor activation.