Project

Exosome biogenesis and the actin cytoskeleton: an exploratory study using bona fide Camelidae nanobodies

Code

01D22115

Duration

01 October 2015 → 30 September 2018

Funding

Regional and community funding: Special Research Fund

Promotor

Jan Gettemans

Fellow

Els Beghein

Research disciplines

Natural sciences
- Biochemistry and metabolism
- Systems biology
Medical and health sciences
- Medical biochemistry and metabolism
- Medical biochemistry and metabolism
- Medical biochemistry and metabolism

Keywords

shRNA Exosome biogenesis nanobody RNAi actin binding proteins single domain antibody heavy chain antibody actin cytoskeleton

Project description

Exosomes are small vesicles released by various types of cells and they influence the behavior of a target cell. Unfortunately, the biogenesis, release and uptake of exosomes is poorly understood. Literature and preliminary data show that the actin cytoskeleton is involved in these processes. Using nanobody and shRNA technologies, we will study the contribution of the cytoskeleton in exosome biogenesis.