Project

Exosome biogenesis and the actin cytoskeleton: an exploratory study using bona fide Camelidae nanobodies

Duration
01 October 2015 → 30 September 2018
Funding
Regional and community funding: Special Research Fund
Research disciplines
  • Natural sciences
    • Biochemistry and metabolism
    • Systems biology
  • Medical and health sciences
    • Medical biochemistry and metabolism
    • Medical biochemistry and metabolism
    • Medical biochemistry and metabolism
Keywords
shRNA Exosome biogenesis nanobody RNAi actin binding proteins single domain antibody heavy chain antibody actin cytoskeleton
 
Project description

Exosomes are small vesicles released by various types of cells and they influence the behavior of a target cell. Unfortunately, the biogenesis, release and uptake of exosomes is poorly understood. Literature and preliminary data show that the actin cytoskeleton is involved in these processes. Using nanobody and shRNA technologies, we will study the contribution of the cytoskeleton in exosome biogenesis.