Code
01D00619
Duration
01 October 2019 → 31 October 2020
Funding
Regional and community funding: Special Research Fund
Promotor
Fellow
Research disciplines
-
Engineering and technology
- Industrial molecular engineering of nucleic acids and proteins
Keywords
Directed evolution
enzyme engineering
glycoside phosphorylase
Project description
Although phosphorylases are attractive biocatalysts for glycoside synthesis, the small number of available specificities (<20) limits their general use. In this project, a mutational strategy will be developed to turn hydrolases into phosphorylases by mimicking their evolutionary pathways. In that way, it should become possible to recruit the much larger diversity of hydrolase specificities (>200) for synthetic purposes.