Project

Combined single-channel electrophysiology and fluorescence microscopy for fundamental studies of ion channel function

Code

01B07618

Duration

01 June 2018 → 31 May 2020

Funding

Regional and community funding: Special Research Fund

Promotor

Luc Leybaert

Research disciplines

Natural sciences
- Medicinal and biomolecular chemistry not elsewhere classified
- Molecular and cell biology not elsewhere classified
- Plant biology not elsewhere classified
- Systems biology not elsewhere classified
Medical and health sciences
- Biophysics not elsewhere classified
- Cell death
- Physiology not elsewhere classified
- Biophysics not elsewhere classified
- Molecular and cell biology not elsewhere classified
- Physiology not elsewhere classified
- Biophysics not elsewhere classified
- Molecular and cell biology not elsewhere classified
- Physiology not elsewhere classified

Keywords

microscopy laser scanning electrophysiology patch-clamp ion channels

Project description

Patch-clamp electrophysiology allows detailed investigations of ion channel function at the level of conformational changes in individual proteins in real-time. Combined with laser-scanning microscopy and fluorescent indicators, the technique allows to monitor additional signals that are involved in ion channel regulation. Overall, the approach is an essential tool for obtaining insight into the physiology/pharmacology of ion channels and receptor-coupled channels.