Exploring oxidative stress responsive post-transcriptional gene regulators in plants

01 October 2019 → 30 September 2022
Regional and community funding: Special Research Fund
Research disciplines
  • Natural sciences
    • Plant cell and molecular biology
Oxidative stress abiotic stresses RNA-binding proteins small RNAs (miRNA/siRNA) transcriptome RBPome small RNA interactome
Project description

Cellular reactive oxygen species (ROS) at low and moderate
concentration can function as second messenger that integrate in the
intra- and intercellular signaling cascades to enable plant acclimation
to abiotic and biotic stresses. Exploiting the key regulators and
targets underlying oxidative stress signaling networks is therefore in
a high priority. It still remains fragmented for comprehensively
identifying gene regulatory mechanisms at the post-transcriptional
level under oxidative stress. Due to the specificity of contemporary
experimental methods, we can systematically detect novel regulators
directly from a native physiological cellular environment. There will be
a comprehensive inventory of functional interactomes for oxidative
stress tolerance generated, which is composed of mRNA interactome
(RBP-transcript) and small RNA interactome (miRNA/siRNAtranscript).
These interactomes can be very helpful to provide the
insights of regulatory processes in oxidative stress signaling
pathways in vivo to understand how the transcripts are regulated at
the post-transcriptional level. Additionally, the novel candidate
regulators identified from the interactomes will be further studied
under diverse abiotic stresses in Arabidopsis plants, the results of
which potentially provides the platform for future engineering of crops
with improved oxidative stress tolerance.