We aim to optimize and validate relevant in vitro biofilm models by testing them with PJI clinical samples and isolates. Our findings will translate into innovative and clinical applicable approaches for a better diagnosis of PJIs and determination of antimicrobial susceptibility profiles of the microorganisms involved in PJIs. To this end, we propose the following specific objectives:

(i) Further development and validation of an in vitro SSF biofilm model and a PJI model;

(ii) Evaluation of the suitability of the SSF and PJI models to support growth and biofilm formation for most significant PJI pathogens;

(iii) Setting up a system for antibiotic susceptibility testing of PJI isolates in both models;

(iv) Setting up a system to use both models to isolate PJI pathogens; and

(v) Validation of the isolation and susceptibility testing protocols using the advanced models and comparing their performance to the current state-of-the-art in clinical practice.