This application for a cost-effective upgrade of an existing spinning disk microscope can be considered as a focussed joint application by 13 ZAP members of our department.

The following molecular cell biology topics will be studied by the different (co-)promotors in the SPINDISK application:

• Regulation of RIPK4-dependent keratinocyte cell adhesion and cytoskeletal dynamics
• Understanding the dynamics of nucleic acid sensing
• Live-cell monitoring of TNFR1 signalling complexes
• Targeting ferroptosis in cancer
• The study of free fatty acid steatosis in the liver in sepsis
• Physiological modelling of cancer cell plasticity using 3D spheroids
• Building frog models for rare diseases and cancer
• Studying dynamic cellular signalling events in NF-kB and inflammasome activation
• Studying the impact of dying cells on clonal competition
• Understanding the molecular mechanisms of phagocytosis
• Studying the interplay between cell death, efferocytosis and wound healing
• Analyzing macrophage heterogeneity in health and disease
• Studying the dynamic cellular interplay in anti-tumour immune responses in the liver.