Project

Single-nanoparticle discrete-charge and size analysis for colloid and life sciences

Acronym
G092017N
Code
3G092017W
Duration
01 January 2017 → 31 December 2020
Funding
Research Foundation - Flanders (FWO)
Research disciplines
  • Natural sciences
    • Soft condensed matter
  • Engineering and technology
    • Nanophotonics
    • Sensors, biosensors and smart sensors not elsewhere classified
Keywords
nanoparticles electrophoresis
 
Project description

Since Millikan’ famous oil drop experiment about hundred years ago, measurements of the electrical charge of single particles with accuracy higher than the elementary charge have been successfully applied in air and in vacuum. More recently, the same concept has been demonstrated by the applicants on single particles in nonpolar liquids. The technique relies on measuring discrete charge levels of a single particle, which allows to determine particle sizes and dynamic changes of size and charge with unprecedented accuracy. The aim of this proposal is to further develop the concept of discrete-charge measurements in liquids that are of high interest for the colloid and life sciences, such as water-based dispersions. A strategy of high-field electrophoresis, dedicated microfluidics and fast optical detection of single nanoparticles is proposed based on insights from protein charge ladders, which demonstrate that stable discrete charges can exist on nano-scale objects. With this work we intend to pioneer and advance the field of single-particle discrete-charge analysis, and ultimately open up a wide variety of possibilities such as single-molecule biosensors, analysis of quantized interactions at the solid-liquid interface, and high-accuracy characterization and identification of viruses and other nanoparticles.