Research Explorer

Project

Detection of DNA-peptide interactions via crosslinking: versatile use of the furan oxidation methodology.
Code
3F007609
Duration
01 October 2009 → 30 September 2013
Funding
Regional and community funding: Special Research Fund, Research Foundation - Flanders (FWO)
Research disciplines
  • Natural sciences
    • Macromolecular and materials chemistry
    • Organic chemistry
Keywords
cross-linking DNA-protein interactions peptide synthesis
 
Project description
 

Through synthesis of major groove binding peptides incorporating an unnatural furan-amino acid building block, we will try to achieve DNA-peptide cross-linking. After complexation of peptide with DNA-duplex, the furan moeity will be oxidized to a reactive enal functionality. Subsequent reaction with the nucleophilic exocyclic amines of DNA bases in major groove will lead to the desired cross-link reaction.