In vitro experiments are often used in research into methane mitigation ration strategies in an initial research phase. However, the linear in vitro - in vivo approach does not provide any indications about the mechanism of action, the kinetics and the possible interactions with the basal ration or the microbial composition in the rumen.

In order to achieve validated reduction percentages more quickly for different animal categories and under various circumstances, we are working with the ILVO on a 'Platform for the Reduction of Enteric Emissions in Cattle' (VLAIO project KlimVEE) based on a more mechanistic and interactive in vitro - in vivo approach . Using scientifically robust in vitro approaches based on in vivo experiments and using artificial intelligence models, critical experimental in vitro and in vivo parameters are identified that should enable extrapolation of emission reductions to various cattle categories, operations, and ration compositions. A better understanding of the mechanism of action should also allow the mitigation potential of combinations to be assessed more quickly, allowing a more limited number and more targeted in vivo tests to be drawn up.