All multicellular organisms are derived from a single cell that divides giving rise to all organs and tissues. This is achieved by asymmetric cell divisions (ACDs) through which daughter cells acquire different fates. Plants undergo organogenesis mainly post-embryonically and therefore, ACD is crucial at both, embryonic and postembryonic stages. Despite its obvious relevance for plant development, many of the components driving ACD remain elusive. Mainly, the ACD taking place in pericycle lateral root founder cells (LRFCs) during lateral root organogenesis is poorly understood to date. To unravel factors contributing to this ACD we propose two complementary approaches. First, a targeted transcript profiling to identify genes differentially regulated in LRFCs upon LR initiation, before and after the first ACD. Second, a chemical genetic screen to uncover additional elements not necessarily regulated at the transcriptional level. In both cases, we will make use of a recently identified signaling peptide involved in the LR initiation process. Excess of GLV6 peptide disrupts the first ACD yielding daughter cells of similar sizes. Therefore GLV6 represents a valuable tool and a new starting point to identify components regulating the ACDs taking place during LR initiation. Besides, we expect to generate a set of new tools including marker lines and chemical compounds useful for futures studies dedicated to gain insights into this developmental process.