Project

Functional validation of cis-regulatory elements of respectively MEF2C and FOXG1

Code
31520518
Duration
01 January 2018 → 31 December 2018
Funding
Research Foundation - Flanders (FWO)
Research disciplines
No data available
Keywords
Rett syndrome
 
Project description

Rett syndrome is a severe neurodevelopmental disorder. In more than 90% of the patients, MECP2

alterations are present. However, in atypical Rett Syndrome, a MECP2 alteration can only be found

in 50-70% of the cases. Alterations in MEF2C and FOXG1 have also been implicated in typical and

atypical Rett Syndrome. Both genes present with a specific spatiotemporal expression pattern. This

strict regulation suggests an important role for regulatory sequences at these loci.

Interestingly, several deletions as well as translocations 5’to MEF2C have been reported in patients

with Rett-like manifestations. The same holds true for FOXG1, where several deletions 3’to FOXG1

have been described in patients with (a)typical Rett Syndrome. As these aberrations solely affect

noncoding regions, this is indicative of deletion/translocation of a crucial regulatory element.

Using 4C-seq we already identified 11 putative regulatory regions for MEF2C (6) & FOXG1 (5). The

aim of this project is to further validate these elements. To do this, we will assess both the in vivo &

in vitro activity pattern of the putative regulatory sequences and determine whether these regions

are transcribed as enhancer RNAs (eRNAs). This will give us valuable information on the regulation

of these genes. This project fits within a larger comprehensive project in which the regulatory

landscape of both genes is fully studied also using other state-of-the-art techniques such as 4C-seq

& CRISPR-Cas9 genome engineering.