Project

Non-viral mediated mRNA based reprogramming of fibroblasts to pluripotent stem cells: overcoming immunogenicity and the need for multiple administrations

Code

3F008614

Duration

01 October 2014 → 30 September 2018

Funding

Regional and community funding: Special Research Fund, Research Foundation - Flanders (FWO)

Promotor

Stefaan De Smedt

Fellow

Joke Devoldere

Research disciplines

Natural sciences
- Biochemistry and metabolism
- Systems biology
Medical and health sciences
- Medical biochemistry and metabolism
- Morphological sciences
- Oncology
- Medical biochemistry and metabolism
- Morphological sciences
- Oncology
- Biomarker discovery and evaluation
- Drug discovery and development
- Medicinal products
- Pharmaceutics
- Pharmacognosy and phytochemistry
- Pharmacology
- Pharmacotherapy
- Toxicology and toxinology
- Other pharmaceutical sciences
- Medical biochemistry and metabolism
- Morphological sciences
- Oncology

Keywords

reprogramming mRNA non-viral gene therapy induced pluripotent stem cells intracellular innate immune cellular barriers

Project description

We aim to optimize mRNA-based reprogramming by a) increasing the time frame of mRNA expression in order to reduce the number of repeated administration, b) decreasing the needed dose of mRNA to avoid immune stimulation and c) inhibition or circumvention of the intracellular innate immune responses. Hence, a fundamental understanding of the intracellular processes which determine protein expression is needed.