Project

Non-viral mediated mRNA based reprogramming of fibroblasts to pluripotent stem cells: overcoming immunogenicity and the need for multiple administrations

Duration
01 October 2014 → 30 September 2018
Funding
Regional and community funding: Special Research Fund, Research Foundation - Flanders (FWO)
Research disciplines
  • Natural sciences
    • Biochemistry and metabolism
    • Systems biology
  • Medical and health sciences
    • Medical biochemistry and metabolism
    • Morphological sciences
    • Oncology
    • Medical biochemistry and metabolism
    • Morphological sciences
    • Oncology
    • Biomarker discovery and evaluation
    • Drug discovery and development
    • Medicinal products
    • Pharmaceutics
    • Pharmacognosy and phytochemistry
    • Pharmacology
    • Pharmacotherapy
    • Toxicology and toxinology
    • Other pharmaceutical sciences
    • Medical biochemistry and metabolism
    • Morphological sciences
    • Oncology
Keywords
reprogramming mRNA non-viral gene therapy induced pluripotent stem cells intracellular innate immune cellular barriers
 
Project description

We aim to optimize mRNA-based reprogramming by a) increasing the time frame of mRNA expression in order to reduce the number of repeated administration, b) decreasing the needed dose of mRNA to avoid immune stimulation and c) inhibition or circumvention of the intracellular innate immune responses. Hence, a fundamental understanding of the intracellular processes which determine protein expression is needed.