Project

Functional analysis of the role of post-translational modifications of TPLATE Complex subunits for clathrin-mediated endocytosis in plants

Code

01SC2815

Duration

01 July 2015 → 30 September 2019

Funding

Regional and community funding: Special Research Fund

Promotor

Eugenia Russinova

Fellow

Jie Wang

Research disciplines

Natural sciences
- General biology
- Plant biology

Keywords

clathrin-mediated endocytosis protein complex purification TPLATE complex plant cell biology

Project description

We recently identified the TPLATE complex as an essential factor for plant clathrin-mediated endocytosis. This complex is evolutionary ancient but apparently lost in ophistokonts. Aim of this project is to unravel the function of post-translational modifications occurring in the subunits of this octameric protein complex. Their functionality will be addressed by live cell imaging, mutant complementation and protein purification assays.