Development of an Orthogonal Expression system in yeast for efficient microbial product synthesis (OrthoExpress)

16 April 2019 → 31 December 2020
Regional and community funding: Industrial Research Fund
Research disciplines
  • Natural sciences
    • Carbohydrates
    • Regulation of metabolism
    • Synthetic biology
    • Genome structure and regulation
    • Microbiology not elsewhere classified
    • Genetics
    • Transcription and translation
    • Genomics
  • Engineering and technology
    • Sustainable and environmental engineering not elsewhere classified
    • Bioprocessing, bioproduction and bioproducts
    • Fermentation
    • Industrial microbiology
    • Other biotechnology, bio-engineering and biosystem engineering not elsewhere classified
Biotechnology Fermentation Sustainable Production Orthogonal Expression System Yeast Human Milk Oligosaccharides
Project description

The main goal of this ConcepTT project is the development and patenting of a generic and independent expression system for (heterologous) genes in yeast to improve (industrial) fermentation processes. This system will act independent (orthogonal) to the native regulation system in yeast by decoupling gene expression from metabolic regulation. Validation of the new technology will occur by the production of the human milk oligosaccharide LNnT as case study.