Project

Screening entire proteomes for yeast-expressable fragments

Duration
01 October 2010 → 30 September 2012
Funding
Regional and community funding: Special Research Fund
Research disciplines
  • Natural sciences
    • Biochemistry and metabolism
    • Systems biology
  • Medical and health sciences
    • Medical biochemistry and metabolism
    • Medical biochemistry and metabolism
    • Medical biochemistry and metabolism
Keywords
yeast surface display coding sequence library eukaryotic secretion system
 
Project description

It is still at present largely unpredictable whether a given protein can be expressed in any production system, including the eukaryotic secretory system. Therefore, we would like to develop a proteome-wide protein fragment "expressability" screening technology in yeast, and manipulate the secretory system to analyze the effect on the "expressable proteome".