Transport processes play a key role in industrial biotechnology processes, one that is however often neglected. This has resulted in deleterious bottlenecks, lower-than-expected bioconversion efficiencies, and potential cytotoxicity. Aquaglyceroporins (AQGPs) are prime candidates to resolve many of these issues, forming simple and substrate specific channels through the membrane that can enhance transport efficiencies for either import or export, while simultaneously being energy neutral. This means that AQGPs can be used in a wide variety of biotechnological processes, making them approach their maximum theoretical efficiency. Building on the experimental information of the determinants of selectivity and natural diversity of AQGPs, precisely designed libraries will be constructed. These will be screened employing a scalable high-throughput biosensor approach, enabled by advances in synthetic biology and generic circuitry. Using these methods, the potential of AQGPs will be harnessed in two proof of concept implementations: to improve selective export of 1,3-propanediol, a key monomer for bioplastics; as well as to boost the import of methanol, a competitive and renewable abiotic feedstock for biotechnology. Success in this project will open the door towards boosting the transport efficiency of small polar molecules across a range of microbial cell factories.