There is a growing need in high-throughput screening assays of 2D/3D cell culture, probing cell viability, biosensor-based imaging, quantifying biomolecules and their spectra, kinetic assays and developing new analytical methods. We intend to purchase the multi-mode fluorescence and time-resolved fluorescence microplate reader, for installation in the Cell and Tissue Culture (CTC2) Core, The Core building, UZ Gent campus. This microplate reader will: (1) expand the sample throughput and capacities of the 10+ research groups using the CTC2 core; (2) enable new assay technologies, such as live monitoring under controlled O₂ levels (hypoxia), assessment of cell energy budget (oxygen consumption rate, extracellular acidification rate, total ATP with various modes of normalisation); (3) support the research of Tissue Engineering and Biomaterials group as well as the other teams working with biomaterial hydrogel-encapsulated cells and tissues under controlled O₂ and pH conditions.