An AFM-nanobiotechnology platform that is composed of (1) a high-speed bio-AFM, and (2) an upgraded AFM with advanced sensors, is proposed. This platform will allow to study various nano(bio)technology topics at a very high state-of-the-art spatial and temporal resolution. The intended research domains include structural biology (microbial adhesins, fibril formation, protein crystallisation), nanomicrobiology (host pathogen interactions, protein-DNA interactions) and microbiology (bacteria and water purification), nanomedicine (cell mechanobiology), and (bio)material science (corrosion, adhesive micropatterns), and food sciences. The system will also be extendedto includes the object manipulation technology that can be used for pen lithography, single cell manipulation, single cell injection, single cell force spectroscopy, and single-cell nanomotion detection. Advanced sensory functionalities based on vibrational and fluorescence microscopy will allow for obtain additional insights into structure of materials and molecule conformation.