Identification and validation of protein substrates and interactors of RIP1 during necrotic cell death

01 October 2008 → 30 September 2010
Regional and community funding: Special Research Fund
Research disciplines
  • Natural sciences
    • Biochemistry and metabolism
    • Systems biology
  • Medical and health sciences
    • Medical biochemistry and metabolism
    • Medical biochemistry and metabolism
    • Medical biochemistry and metabolism
  • Engineering and technology
    • Biomaterials engineering
    • Biological system engineering
    • Biomaterials engineering
    • Biomechanical engineering
    • Other (bio)medical engineering
    • Environmental engineering and biotechnology
    • Industrial biotechnology
    • Other biotechnology, bio-engineering and biosystem engineering
kinase substrates RIP1 necrosis
Project description

Necrotic cell death occurs during viral infection, organ transplantations and infarction, and is dependent on the kinase-activity of Receptor Interacting Protein (RIP1).The aim of this project is to identify specific substrates (phospho-specific MS analysis and peptide-array) and interactors (co-immunoprecipitation of RIP1 from necrotic cells) of RIP1. These experiments will provide us with a better insight in the necrotic signal transduction cascade.