Project

Use of fluorescent techniques for cytogenetic characterization of Araceae somatic hybridization products

Duration
01 February 2009 → 31 July 2012
Funding
Regional and community funding: Special Research Fund
Research disciplines
  • Natural sciences
    • Plant biology
  • Engineering and technology
    • Biomaterials engineering
    • Biological system engineering
    • Biomaterials engineering
    • Biomechanical engineering
    • Other (bio)medical engineering
    • Environmental engineering and biotechnology
    • Industrial biotechnology
    • Other biotechnology, bio-engineering and biosystem engineering
  • Agricultural and food sciences
    • Agricultural plant production
    • Horticultural production
Keywords
asymmetric hybrids GISH chromosome visualization protoplast fusion interspecific microprotoplasts
 
Project description

The aim is to create asymmetric somatic hybrids within the Araceae family, and to visualize (partial) chromosome transfer of the donor to the acceptor genome. Genome fragmentation will be induced through microprotoplasts isolation or UV-irradiation and fusion will ne performed either chemically or electrically. A proper GISH protocol will be designed for cytogenetic testing of the obtained hybrids.